Thy1 chr2 yfp

Author: pubv

August undefined, 2024

Webb9 sep. 2015 · The mouse line used (Thy1::ChR2-YFP line 4 and Thy1::eNpHR-YFP line 4) was purchased from The Jackson Laboratory. Throughout the experiment, the mouse was maintained under deep anesthesia and its body temperature was maintained at 37.5 °C. Webb15 jan. 2024 · One of the first transgenic mouse lines developed for the optogenetic …

Optogenetic stimulation of mouse Hoxb8 microglia in specific …

Webb25 okt. 2024 · Hernandez et al. (2014) elicited light-evoked responses from the peripheral auditory system in Thy1.2-ChR2-YFP transgenic mice expressing ChR2 in spiral ganglion neurons (SGNs) of the cochlea. Overall, these studies demonstrate potential for optogenetic activation of the auditory system from the periphery, where SGNs comprise … WebbThy1-channelrhodopsin-2 (Thy1-ChR2-eYFP) and Thy1-YFP lines mark a population of glutamatergic pyramidal neurons within the BLA, with signiﬁcant speciﬁcity for the anterior basal amygdala16. Optogenetic activation of this population leads to the inhibition of a population of central amygdala (CeA) neurons and shunts excitatory drive from the elders aboriginal

初级听觉皮层预测相关神经机制--《第三军医大学》2024年博士论文

Webb2 dec. 2024 · University of California San Diego, La Jolla, California, United States . Background: Human brain functions, including perception, attention, and other higher-order cognitive functions, are supported by neural oscillations necessary for the transmission of information across neural networks. Previous studies have demonstrated that the … Webb20 juli 2016 · Control experiments in Thy1/ChR2-YFP mice showed that CSD was not induced by pulses of red (630 nm) light that do not activate ChR2 or by light-induced rises in cortical temperature (Supplementary Figure 3(a) to (c)). Characteristics of CSD change when repeated after 20 min. Webb6 apr. 2024 · We then applied 32-day patterned optogenetic stimulation of SuM neurons in 3.5-month-old triple transgenic Ascl1-Ai9-AD mice injected with AAV-CaMKII-ChR2-YFP or AAV-CaMKII-YFP in the SuM, referred to as ChR2-AD (experimental) or YFP-AD (control) mice (Figures 2 H and 2I), followed by the quantification of the number and … elders ace hardware chapman highway

Thy1 chr2 yfp

Transient, Consequential Increases in Extracellular Potassium …

WebbMapping Anatomy to Behavior in Thy1:18 ChR2-YFP Transgenic Mice Using Optogenetics. In Molecular Neuroscience: A Laboratory Manual 594-606. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press. Webb19 juni 2013 · Thy1-promoter (Thy1-YFP; Thy1-ChR2-EYFP), which target a spe- cific subpopulation of pyramidal neurons within amygdala and other forebrain areas ( Sugino et al., 2006 ).

Did you know?

Webb007615 B6.Cg-Tg(Thy1-COP4/EYFP)9Gfng/J Tg(Thy1-COP4/EYFP)9Gfng (or Thy1-ChR2 … Webb26 nov. 2024 · One of the first transgenic mouse lines developed for the optogenetic …

Webb1 juni 2015 · Here, we describe the use of transgenic mice expressing the blue-light gated … WebbFurthermore, using Thy1-ChR2-YFP mice we demonstrate the application of our probes in simultaneous recording and optical/chemical modulation of brain activities across distant regions.

WebbRecombinant DNA technologies have enabled the development of transgenic animal models for use in studying a myriad of diseases and biological states. … Webb10 apr. 2024 · Showing that YFP is expressed necessarily means that ChR2 is also expressed, because the YFP and ChR2 are present in Ai32 as a fusion gene leading to production of a YFP/ChR2 fusion protein, both ...

Webb21 okt. 2016 · Thy1-channelrhodopsin-2 (Thy1-ChR2-eYFP) and Thy1-YFP lines mark a …

Webb22 dec. 2024 · Model 1: activation of both pyramidal cells (Pyrs) and interneurons (INs) expressing Channelrhodopsin2 (ChR2) with 470 nm of photostimulation. ChR2 is a nonspecific cation channel that opens when exposed to light. This results in depolarization and the generation of action potentials in the neurons. food inventory spreadsheet excelWebb(a) Expression of channelrhodopsin-2 (ChR2) in Thy1-ChR2 mice. (b) Preferential expression of YFP-tagged ChR2 in layer 5 pyramidal neurons in the cortex. The image in the zoomed image is... food inventory systemWebb15 jan. 2024 · The Thy1-ChR2 transgenic mouse is commonly used in optogenetic … food inventory spreadsheet for restaurantsWebb19 juni 2013 · We first molecularly characterized Thy1-channelrhodopsin-2 (Thy1-ChR2 … food inventory spreadsheet templateWebb20 feb. 2024 · The probe was lowered until an intense spiking band was found, which covered 30–40% of electrode sites near the correct ventral coordinate, thus reflecting the piriform layer II. For the optogenetic experiments stimulating OB cells in Thy1 ChR2/ChR2-YFP mice, the optic fiber was placed <500 µm above the OB dorsal surface. food inventory tracking softwareWebbRecently, a ratiometric CFP-YFP based construct, termed “Cl-Sensor”, with a relatively high sensitivity to Cl has been proposed (Markova et al., 2008). In the present study, we have developed conditions for the efficient expression of Cl-Sensor in tiny neuronal compartments including distal dendrites and spines. elders ace hardware corporate officeWebbMice hemizygous for the TpH2-ChR2-YFP BAC transgene are viable and fertile, with expression of the mhChR2::YFP fusion protein directed to serotonergic neuronal populations by the mouse tryptophan hydroxylase 2 (Tph2 or TpH2) promoter/enhancer regions on the BAC transgene.The mhChR2::YFP fusion protein is composed of a … elders ace hardware chickamauga