Webpurchased from Fisher Scientific (Schwerte, Germany). Water (LC/MS grade) and boron trifluoride (BF 3) (14 % in methanol) were purchased from Sigma-Aldrich (Hamburg, Germany). Natriumsulfate (Na 2 SO 4) was purchased from Merck (Darmstadt, Germany). A certified 37 fatty acid methyl ester (FAME) mix (TraceCERT) Web58239 Schwerte Fon: 02304 932 5 Fon: 02304 932 350 Email: [email protected] Allgemeine Laborgeräte, Automation und Integration …
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WebMay 6, 2024 · To determine the protein concentration of the secretome and proteome samples, dilute the secretome samples 1:6 and the proteome samples 1:11 in water to reach a concentration of about 0.5–1 μg/μL and use the Pierce 660 nm Protein Assay (Fisher Scientific, Schwerte, Germany) (see Note 9). 2. WebApr 8, 2024 · The RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific GmbH, Schwerte, Germany) was used for cDNA synthesis according to the provided protocol, and the cDNA concentration and purity were determined by UV photometry. qPCR analysis was performed by using the StepOne Plus Real-Time PCR System (Thermo … how many professional engineers in usa
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WebFeb 25, 2024 · After 24 h, THP-1 cells were fluorescence-labeled with CellTracker Green (Thermo Fisher Scientific, Schwerte, Germany), and 100,000 cells per well were allowed to adhere onto an endothelial cell monolayer. The amount of adherent THP-1 or Jurkat cells was measured at 485 nm (ex) and 535 nm (em) using a plate reader (SPECTRAFluor … WebFinden Sie jetzt 12 zu besetzende Unity Jobs in Reh auf Indeed.com, der weltweiten Nr. 1 der Online-Jobbörsen. (Basierend auf Total Visits weltweit, Quelle: comScore) WebPOC was determined using an elemental analyzer (Thermo Flash 2000; Thermo Fisher Scientific, Schwerte, Germany) after Sharp (1974). FAs were measured as FA methyl esters (FAMEs) using a gas chromatograph (Trace GC Ultra; Thermo Fisher Scientific, Schwerte, Germany). Analytical procedure of FAs was modified after Christie (1989). how cpk is calculated